Template Switch Oligo
Use this set of oligonucleotides for template switching cdna synthesis and preamplification. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The tso adds a common 5'. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The resulting cdna can be amplified by pcr or serve as. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2.
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Template Switch Oligo
We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains a portion. The tso adds a common 5'. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor.
Template Switch Oligo
The resulting cdna can be amplified by pcr or serve as. We recommend a tso with rgrgrg at the 3′ end. The tso adds a common 5'. However, if a large fraction of the library contains a portion. A small fraction of visium libraries are expected to contain the template.
Template Switch Oligo Printable Word Searches
We recommend a tso with rgrgrg at the 3′ end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The resulting cdna can be amplified by pcr or serve as. The tso (template switch oligo) is an oligo.
Template Switch Oligo Printable Word Searches
Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. A small fraction of.
Template Switch Oligo
In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The resulting cdna can be amplified by pcr or serve as. The tso adds a common 5'. However, if a large fraction of the library contains a portion. In conjunction.
Template Switch Oligo Printable Word Searches
The resulting cdna can be amplified by pcr or serve as. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. We recommend a tso with rgrgrg at the 3′ end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In general, different tsos can.
The Resulting Cdna Can Be Amplified By Pcr Or Serve As.
However, if a large fraction of the library contains a portion. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. We recommend a tso with rgrgrg at the 3′ end.
Use This Set Of Oligonucleotides For Template Switching Cdna Synthesis And Preamplification.
The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. The tso adds a common 5'. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end.